國家衛生研究院 NHRI:Item 3990099045/10039
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    题名: Cross-reactive neutralizing antibody responses of high-growth enterovirus 71 genotype B5 vaccine candidate in rabbits
    作者: Chia, MY;Chung, WY;Wang, CH;Chang, WH;Lee, MS
    贡献者: Division of Infectious Diseases
    摘要: Enterovirus 71 (EV71) is a non-enveloped RNA virus of the family Picornaviridae. Based on phylogenetic analysis of the most variable VP1 gene, EV71 could be classified into 3 major genogroups (A, B and C) including 11 genotypes (A, B1~B5, and C1~C5). Since 1997, different EV71 genotypes have caused life-threatening epidemics with severe neurologic complications in Asia, including Malaysia, Taiwan, Singapore, Brunei, Vietnam, Cambodia and China. Therefore, development of EV71 vaccines is a national priority in these countries. Currently, five vaccine candidates have been evaluated in clinical trials in China (3 genotype C4 candidates), Singapore (1 genotype B2 candidate), and Taiwan (1 genotype B4 candidate). Overall, these 5 vaccine candidates could not grow very well (~107 PFU/ml) in cells qualified for vaccine production. In addition, genotypes of these 5 candidates are different from the current predominant genotype B5 in Taiwan and South-Eastern Asia. Therefore, we generated high-growth EV71 genotype B5 virus (HG-B5) which could grow to high titers (~108 PFU/ml) in Vero cells. Infectious HG-B5 particles were purified using sucrose gradient ultracentrifugation and collected to immunize rabbits. Rabbits (two rabbits for each group) were intramuscularly immunized with two doses of purified HG-B5 at 0 and 14 day post-immunization (DPI) using two dosages (0.05 and 0.25 ug of total protein) adjuvanted with alum. The purified HG-B5 could induce cross-reactive neutralizing antibody responses against three EV71 major genogroups. The results indicate that Vero cell-derived HG-B5 vaccine candidate is immunogenic in rabbits and has commercial potential for development of new-generation EV71 vaccines.
    日期: 2016-08
    關聯: European Journal of Immunology. 2016 Aug;46(Suppl. 1):324.
    Link to: http://dx.doi.org/10.1002/eji.201670200
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0014-2980&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000383610400653
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