國家衛生研究院 NHRI:Item 3990099045/10047

NHRI > Center for Neuropsychiatric Research > Hwei-Hisen Chen > Conference Papers/Meeting Abstract > Item 3990099045/10047

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/10047

Title:	Extracellular calcium concentration and channel activity involved in aminoglycoside-induced hair cell toxicity in the zebrafish lateral line
Authors:	Chan, MH;Wu, CY;Liu, CF;Chen, HH
Contributors:	Center for Neuropsychiatric Research
Abstract:	Introduction: Ototoxic damage often caused by antibiotic aminoglycosides leads to the loss of hair cells. Since aminoglycosides are effective at infection treatment, it is imperative to discover new approach to prevent hearing loss and permit safe use of aminoglycosides. The alterations in cytosolic calcium homeostasis play an important role in aminoglycoside-induced sensory hair cell death. However, the involvement of cytoplasmic membrane calcium channels in aminoglycoside ototoxicity is not clear. Objective: The present study determined the role of calcium and its channel activity in ototoxicity induced by aminoglycosides. Materials and methods: In this study, the lateral lines of zebrafish were used to investigate the effects of verapamil, a calcium channel blocker, and Bay K8644, a calcium channel activator, on aminoglycoside (neomycin and gentamycin)-induced hair cell toxicity which was detected in vivo by the dye FM1-43FX, a reliable indicator to assess hair cell viability. Results: The data showed that verapamil significantly attenuated both aminoglycosides induced hair cell loss, whereas Bay K8644 enhanced their ototoxicity. Interestingly, the loss of hair cells induced by both aminoglycosides was respectively reduced and reinforced under extracellular high calcium (3.3 mM) and low calcium (33 μM) concentrations compared with normal calcium (330 μM) condition. Furthermore, Bay K8644 partly inhibited the protective action of extracellular high calcium on hair cell death mediated by the aminoglycoside treatment. Conclusions: These findings indicate that both extracellular calcium concentration and calcium channel activity influence hair cell loss from aminoglycoside toxicity.
Date:	2016-10-10
Relation:	Toxicology Letters. 2016 Oct 10;259(Suppl.):S202.
Link to:	http://dx.doi.org/10.1016/j.toxlet.2016.07.484
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0378-4274&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000396848100502
Appears in Collections:	[Hwei-Hisen Chen] Conference Papers/Meeting Abstract

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