國家衛生研究院 NHRI:Item 3990099045/10122

NHRI > National Institute of Infectious Diseases and Vaccinology > Ming-Hsi Huang > Periodical Articles > Item 3990099045/10122

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/10122

Title:	Degradable emulsion as vaccine adjuvant reshapes antigen-specific immunity and thereby ameliorates vaccine efficacy
Authors:	Huang, CH;Huang, CY;Cheng, CP;Dai, SH;Chen, HW;Leng, CH;Chong, P;Liu, SJ;Huang, MH
Contributors:	Division of Vaccine Research and Development
Abstract:	This study describes the feasibility and adjuvant mechanism of a degradable emulsion for tuning adaptive immune responses to a vaccine antigen. We featured a mouse model with ovalbumin (OVA) as the antigen to deepen our understanding of the properties of a degradable emulsion-based adjuvant, dubbed PELC, interacting with immune cells and to elucidate their roles in vaccine immunogenicity in vivo. First, we demonstrated that the emulsion, which is stabilized by an amphiphilic bioresorbable polymer, shows degradation in mimic human body conditions and considerable tolerance in vivo. Then, we confirmed the model protein could be loaded into the emulsion and released from the matrix in a sustained manner, subsequently driving the production of antigen-specific antibodies. We also comprehended that PELC not only recruits antigen-presenting cells (APCs) to the injection site but also induces the activation of the recruited APCs and migration to the draining lymph nodes. As an adjuvant for cancer immunotherapy, PELC-formulated OVA could strongly enhance antigen-specific T-cell responses as well as anti-tumor ability with respected to non-formulated OVA, using OVA protein/EG7 cells as a tumor antigen/tumor cell model. Accordingly, our data paved the way for the clinical application of degradable emulsions based on amphiphilic bioresorbable polymers as vaccine adjuvants.
Date:	2016-11-09
Relation:	Scientific Reports. 2016 Nov 09;6:Article number 36732.
Link to:	http://dx.doi.org/10.1038/srep36732
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=2045-2322&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000387540500001
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84994518217
Appears in Collections:	[Ming-Hsi Huang] Periodical Articles [Shih-Jen Liu] Periodical Articles [Pele Choi-Sing Chong] Periodical Articles [Chih-Hsiang Leng] Periodical Articles [Hsin-Wei Chen] Periodical Articles

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