國家衛生研究院 NHRI:Item 3990099045/10304
English  |  正體中文  |  简体中文  |  全文笔数/总笔数 : 12189/12972 (94%)
造访人次 : 953943      在线人数 : 696
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜寻范围 查询小技巧：
  • 您可在西文检索词汇前后加上"双引号"，以获取较精准的检索结果
  • 若欲以作者姓名搜寻，建议至进阶搜寻限定作者字段，可获得较完整数据
    •  进阶搜寻
    主页 ‧ 登入 ‧ 上传 ‧ 说明 ‧ 关于NHRI ‧ 管理 到手机版


    jsp.display-item.identifier=請使用永久網址來引用或連結此文件: http://ir.nhri.org.tw/handle/3990099045/10304


    题名: Statins protect airway epithelium against bacterial pore-forming toxins
    作者: Kao, CY;Hung, LY;Ruan, JW;Chang, CY;Lim, JH;Li, JD;Wu, R
    贡献者: Immunology Research Center
    摘要: Infectious diseases remain a leading cause of morbidity and mortality worldwide. Antibiotics are used to treat many common forms of bacterial infections. However, the discovery of new classes of antibiotics has lagged behind in the last several decades, and yet the emergence of multi-drug resistant bacterial strains due to the widespread overuse of antibiotics is a growing problem. There is a critical need for antibacterial agents not subject to currently observed resistance mechanisms. Pneumonia kills approximately two million children each year worldwide and millions of people get pneumonia annually in the United States alone. Airway epithelia are the initial and primary sites of many bacterial contacts and their barrier and immunity functions are critical to fight infections. Using primary normal human bronchial epithelial (NHBE) cells and an immortalized normal bronchial epithelial cell line, HBE1, here we show that the prior exposure to statins confers significant cellular resistance to the cytotoxicity of pneumolysin and -hemolysin. Pneumolysin and -hemolysin are the main pore-forming toxins, the single largest class of bacterial virulence factors, shown to play critical roles in bacterial pathogenesis respectively in Streptococcus and , two common causes of bacterial infections. We further found that the protection requires protein pneumoniae Staphylococcus aureus synthesis and is calcium dependent. With siRNA gene silencing, pharmacological inhibitors, immuno fluorescence microscopy and biochemical approaches, we have delineated the statin-mediated cellular protection mechanisms in airway epithelial cells. Our animal experiment results further suggest that statin pre-treatment could reduce the lung inflammation caused by pneumolysin. The ultimate goal of this study is to elucidate the interactions of pore-forming toxins and the host cells at the molecular level and this might be potentially developed as a novel host-directed adjuvant therapy in pore-forming toxin-related bacterial infections.
    日期: 2013-05-19
    關聯: American Journal of Respiratory and Critical Care Medicine. 2013 May 19;187:Meeting Abstract A6095.
    Link to: http://www.atsjournals.org/doi/pdf/10.1164/ajrccm-conference.2013.187.1_MeetingAbstracts.A6095
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1073-449X&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000209838403085
    显示于类别:[其他] 會議論文/會議摘要

    文件中的档案:

    档案 描述 大小格式浏览次数
    ISI000209838403085.pdf16KbAdobe PDF383检视/开启


    在NHRI中所有的数据项都受到原著作权保护.

    TAIR相关文章

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回馈