國家衛生研究院 NHRI:Item 3990099045/10365

NHRI > National Institute of Infectious Diseases and Vaccinology > Pele Choi-Sing Chong > Periodical Articles > Item 3990099045/10365

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/10365

Title:	Enhancing enterovirus A71 vaccine production yield by microcarrier profusion bioreactor culture
Authors:	Liu, CC;Wu, SC;Wu, SR;Lin, HY;Guo, MS;Hu, AYC;Chow, YH;Chiang, JR;Shieh, DB;Chong, P
Contributors:	Division of Vaccine Research and Development
Abstract:	Hand, foot and mouth diseases (HFMD) are mainly caused by Enterovirus A71 (EV-A71) infections. Clinical trials in Asia conducted with formalin-inactivated EV-A71 vaccine candidates produced from serum-free Vero cell culture using either roller bottle or cell factory technology, are found to be safe and highly efficacious. To increase vaccine yields and reduce the production costs, the bioprocess improvement for EV-A71 vaccine manufacturing is currently being investigated. The parameters that could affect and enhance the production yields of EV-A71 virus growth in the microcarrier bioreactor were investigated. The medium replacement culture strategy included a multi-harvested semi-batch process and perfusion technology and was found to increase the production yields more than 7-14 folds. Based on the western blot and cryo-EM analyses of the EV-A71 virus particles produced from either the multi-harvested semi-batch (MHSBC) or perfusion cultures were found to be similar to those virus particles obtained from the single batch culture. Mouse immunogenicity studies indicate that the EV-A71 vaccine candidates produced from the perfusion culture have similar potency to those obtained from single batch bioprocess. The physical structures of the EV-A71 particles revealed by the cryo-EM analysis were found to be spherical capsid particles. These results provide feasible technical bioprocesses for increasing virus yields and the scale up of EV-A71 vaccine manufacturing using the bioreactor cell culture methods.
Date:	2018-05
Relation:	Vaccine. 2018 May;36(22):3134-3139.
Link to:	http://dx.doi.org/10.1016/j.vaccine.2017.02.042
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0264-410X&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000433647600009
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85014506979
Appears in Collections:	[Pele Choi-Sing Chong] Periodical Articles [Joe Yen-Hung Chow] Periodical Articles [Yung-Chih Hu] Periodical Articles [Chia-Chyi Liu] Periodical Articles

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