國家衛生研究院 NHRI:Item 3990099045/10434
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    题名: In vivo inhibition of influenza A virus replication by RNA interference targeting the PB2 subunit via intratracheal delivery
    作者: Huang, DTN;Lu, CY;Shao, PL;Chang, LY;Wang, JY;Chang, YH;Lai, MJ;Chi, YH;Huang, LM
    贡献者: Institute of Biotechnology and Pharmaceutical Research
    摘要: Background Influenza virus infection is a major threat to human health. Small interfering RNA (siRNA) is a promising approach for the prevention and treatment of viral infections. In this study, we constructed a series of DNA vector-based short hairpin RNAs (shRNAs) that target various genes of the influenza A virus using the polymerase III U6-RNA promoter to prevent influenza virus infection in vitro and in a mouse model. Results Three sets of DNA vector-based shRNA, two targeting genes encoding the polymerase acidic protein (PA) and one targeting polymerase basic protein 2 (PB2), efficiently inhibited the replication of influenza virus A/WSN/33(H1N1) in vitro. We also successfully prevented influenza virus A/WSN/33(H1N1) infection in a C57BL/6 mouse model by intratracheal delivery of anti-PB2 shRNA. Conclusions Our findings suggest that the PB2-targeting shRNA plasmid showed potential for use as an RNAi-based therapeutic for influenza virus infection.
    日期: 2017-04-05
    關聯: PLoS ONE. 2017 Apr 5;12(4):Article number e0174523.
    Link to: http://dx.doi.org/10.1371/journal.pone.0174523
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1932-6203&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000399353500051
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85016944229
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