國家衛生研究院 NHRI:Item 3990099045/10499
English  |  正體中文  |  简体中文  |  全文笔数/总笔数 : 12145/12927 (94%)
造访人次 : 855595      在线人数 : 1242
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜寻范围 查询小技巧：
  • 您可在西文检索词汇前后加上"双引号"，以获取较精准的检索结果
  • 若欲以作者姓名搜寻，建议至进阶搜寻限定作者字段，可获得较完整数据
    •  进阶搜寻
    主页 ‧ 登入 ‧ 上传 ‧ 说明 ‧ 关于NHRI ‧ 管理 到手机版


    jsp.display-item.identifier=請使用永久網址來引用或連結此文件: http://ir.nhri.org.tw/handle/3990099045/10499


    题名: Monitoring ER calcium and ER proteostasis under hypoxic conditions
    作者: Trychta, KA;Yan, X;Anttila, J;Airavaara, M;Wu, KJ;Wang, Y;Henderson, MJ;Harvey, BK
    贡献者: Center for Neuropsychiatric Research
    摘要: Under normal conditions, the calcium concentration in the lumen of the endoplasmic reticulum (ER) is 1,000 to 10,000 times higher than in the cytoplasm. ER calcium is important for many cellular functions, such as protein folding, lipid metabolism, and signaling pathways. Disruption of ER calcium homeostasis is implicated in multiple neurological diseases, including stroke. We used secreted ER calcium modulated proteins (SERCaMPs) to monitor ER calcium depletion in models of stroke. Using a previously described Gaussia luciferase (GLuc)-SERCaMP in vitro, we demonstrate here that oxygen-glucose deprivation increased ER calcium depletion and decreased cell viability. Additionally, we developed a series of GLuc-based SERCaMP reporters representing ~80 ER resident proteins and observed a widespread shift in the reporters following ischemia, which suggests changes to the ER proteome. This increase in SERCaMP response was attenuated by treatment with the ryanodine receptor (RyR) antagonist dantrolene, which also attenuated ER calcium depletion and increased cell viability. Rats injected with adeno-associated virus (AAV) vectors expressing GLucSERCaMP in the cortex showed evidence of ER calcium depletion following occlusion of the middle cerebral artery. Furthermore, dantrolene treatment reduced the infarct volume in rats. Collectively, our data support a model in which ischemic injury causes ER calcium depletion and the secretion of important ER resident proteins. These effects can be attenuated by stabilizing ER calcium and preventing loss of ER resident proteins.
    日期: 2017-04-04
    關聯: Cell Transplantation. 2017 Apr 4;26(4, Suppl.1):728-729.
    Link to: https://doi.org/10.3727/096368917X695146
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0963-6897&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000416626200076
    显示于类别:[王昀] 會議論文/會議摘要

    文件中的档案:

    档案 描述 大小格式浏览次数
    ISI000400124700090.pdf72KbAdobe PDF402检视/开启


    在NHRI中所有的数据项都受到原著作权保护.

    TAIR相关文章

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回馈