 |
English
|
正體中文
|
简体中文
|
Items with full text/Total items : 12145/12927 (94%)
Visitors : 851499
Online Users : 841
|
|
|
Loading...
|
Please use this identifier to cite or link to this item:
http://ir.nhri.org.tw/handle/3990099045/10579
|
| Title: | Chronic treatment with cisplatin induces chemoresistance through the TIP60-mediated Fanconi anemia and homologous recombination repair pathways |
| Authors: | Su, WP;Ho, YC;Wu, CK;Hsu, SH;Shiu, JL;Huang, JC;Chang, SB;Chiu, WT;Hung, JJ;Liu, TL;Wu, WS;Wu, PY;Su, WC;Chang, JY;Liaw, H |
| Contributors: | National Institute of Cancer Research |
| Abstract: | The Fanconi anemia pathway in coordination with homologous recombination is essential to repair interstrand crosslinks (ICLs) caused by cisplatin. TIP60 belongs to the MYST family of acetyltransferases and is involved in DNA repair and regulation of gene transcription. Although the physical interaction between the TIP60 and FANCD2 proteins has been identified that is critical for ICL repair, it is still elusive whether TIP60 regulates the expression of FA and HR genes. In this study, we found that the chemoresistant nasopharyngeal carcinoma cells, derived from chronic treatment of cisplatin, show elevated expression of TIP60. Furthermore, TIP60 binds to the promoters of FANCD2 and BRCA1 by using the chromatin immunoprecipitation experiments and promote the expression of FANCD2 and BRCA1. Importantly, the depletion of TIP60 significantly reduces sister chromatid exchange, a measurement of HR efficiency. The similar results were also shown in the FNACD2-, and BRCA1-deficient cells. Additionally, these TIP60-deficient cells encounter more frequent stalled forks, as well as more DNA double-strand breaks resulting from the collapse of stalled forks. Taken together, our results suggest that TIP60 promotes the expression of FA and HR genes that are important for ICL repair and the chemoresistant phenotype under chronic treatment with cisplatin. |
| Date: | 2017-06-20 |
| Relation: | Scientific Reports. 2017 Jun 20;7:Article number 3879. |
| Link to: | http://dx.doi.org/10.1038/s41598-017-04223-5 |
| JIF/Ranking 2023: | http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=2045-2322&DestApp=IC2JCR |
| Cited Times(WOS): | https://www.webofscience.com/wos/woscc/full-record/WOS:000403643900032 |
| Cited Times(Scopus): | https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85021088113 |
| Appears in Collections: | [張俊彥] 期刊論文
|
Files in This Item:
| File |
Description |
Size | Format | |
|---|
| PUB28634400.pdf | | 4929Kb | Adobe PDF | 335 | View/Open |
|
All items in NHRI are protected by copyright, with all rights reserved.
|
