國家衛生研究院 NHRI:Item 3990099045/10596
English  |  正體中文  |  简体中文  |  全文笔数/总笔数 : 12145/12927 (94%)
造访人次 : 853366      在线人数 : 853
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜寻范围 查询小技巧：
  • 您可在西文检索词汇前后加上"双引号"，以获取较精准的检索结果
  • 若欲以作者姓名搜寻，建议至进阶搜寻限定作者字段，可获得较完整数据
    •  进阶搜寻
    主页 ‧ 登入 ‧ 上传 ‧ 说明 ‧ 关于NHRI ‧ 管理 到手机版


    jsp.display-item.identifier=請使用永久網址來引用或連結此文件: http://ir.nhri.org.tw/handle/3990099045/10596


    题名: Zebrafish nicastrin deficiency leads to a Tyrosinase-dependent depigmentation
    作者: Hsu, CH;Jiang, YJ
    贡献者: Institute of Molecular and Genomic Medicine
    摘要: Notch is a highly conserved signaling pathway involving in adjacent cell regulations, such as differentiation, proliferation and apoptosis. The perturbation of the signaling pathway is associated with cardiac diseases, intrahepatic bile duct paucity, inflammatory diseases, and also skeletal patterning defects. Recently, suppressing of Notch signaling by γ-secretase inhibitor was also found to alter the patterning of melanocytes on adult zebrafish and induce hair graying on mice with mechanism remained largely unknown. To understand the potential role of γ-secretase in pigment regulation, nicastrin hi1384 , which has an insertional mutation on one of theγ-secretase components, was employed. Here we found that, the nicastrin hi1384 mutant, which has a GT2.0 virus insertion in the intron one region and, therefore, loses the expression of nicastrin mRNA, showed hypopigmentation and curly up tail phenotypes. Besides, the phenotypes can be partially mimicked or rescued in morpholino knockdown experiments and nicastrin mRNA overexpression, respectively. With live image observation and whole mount in situ hybridization, the melanocytes and eye iridophores were found gradually losing in the homozygotes. The cone and rod cells were also vanishing in the progression of depigmentation according to H&E staining. Through literature research, we were suspecting that the depigmentation might be caused by toxic components, including Eumelanin and ROS, produced by Tyrosinase. The Tyrosinase inhibitor and ROS inhibitor were, therefore, applied to test the hypothesis, and the results suggest that Eumelanin could be the toxic component to induce Vitiligo in the nicastrin mutant.
    日期: 2017-07
    關聯: Mechanisms of Development. 2017 Jul;145(Suppl.):S99.
    Link to: https://doi.org/10.1016/j.mod.2017.04.255
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000402994800328
    显示于类别:[江運金] 會議論文/會議摘要

    文件中的档案:

    档案 描述 大小格式浏览次数
    ISI000402994800328.pdf45KbAdobe PDF355检视/开启


    在NHRI中所有的数据项都受到原著作权保护.

    TAIR相关文章

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回馈