國家衛生研究院 NHRI:Item 3990099045/10956
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    题名: Stromal C-type lectin receptor COLEC12 integrates H. pylori, PGE2-EP2/4 axis and innate immunity in gastric diseases
    作者: Chang, LL;Hsu, WH;Kao, MC;Chou, CC;Lin, CC;Liu, CJ;Weng, BC;Kuo, FC;Kuo, CH;Wang, CJ;Lin, CH;Wu, DC;Huang, SK
    贡献者: National Institute of Environmental Health Sciences
    摘要: Background: Tissue stroma is known to be important in regulating Hp-mediated inflammation, but the molecular basis of its interaction with Hp and its potential crosstalk with dendritic cells (DCs) remain to be determined. To this end, the potential crosstalk between H. pylori (Hp) infected gastric stromal cells (Hp-GSCs) and DCs were investigated. Methods: The expression of IL-23 and IL-17 indicative activation of DCs and Th17 cells, respectively, was evaluated as the primary experimental readouts. Primary CD90 + GSCs from cancerous and adjacent normal tissues were generated from gastric cancer patients, and monocyte-derived DCs were obtained from healthy individuals. Levels of cytokines and prostaglandin E 2 (PGE 2 ) were measured by ELISA, and C-type lectin expression in GSCs was assessed by flow cytometry and immunohistochemistry. Results: In a trans-well co-culture system, significantly upregulated DC-derived IL-23 expression was found when DCs were cocultured with Hp-infected GSCs (Hp-GSCs). Further, PGE 2 from Hp-GSCs was discovered to possess the priming effect, which could be inhibited, by anti-COLEC12 (Collectin subfamily member 12) blocking Abs, COLEC12 knockdown or when alpha3-fucosyltrans-ferase-null ( futB; HP0651) strain of Hp was used. Also, the expression of COLEC12 was co-localized with CD90+ stromal cells in cancerous tissues. Furthermore, Hp-GSCs-conditioned DCs were able to induce the expression of IL-17 from CD4+ T cells, which could be inhibited by IL-23-neutralizing Abs. Conclusion: These results suggested the importance of COLEC12 as a receptor involved in Hp-stromal cell interaction and its subsequent conditioning effect on DCs.
    日期: 2017-11
    關聯: International Journal of Antimicrobial Agents. 2017 Nov;50(Suppl. 2):S87.
    Link to: http://dx.doi.org/10.1016/S0924-8579(17)30423-5
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0924-8579&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000416028900196
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