國家衛生研究院 NHRI:Item 3990099045/11011

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Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/11011

Title:	Rapid measurement of fluorescence lifetimes using SIPM detection and waveform sampling
Authors:	Tsai, HM;Souris, JS;Kim, HJ;Cheng, SH;Chen, CT;Lo, LW;Chen, L;Kao, CM
Contributors:	Institute of Biomedical Engineering and Nanomedicine
Abstract:	In this work we demonstrate, for the first time, the feasibility of making rapid fluorescence lifetime measurements with analog silicon photomultipliers (SiPMs) and waveform sampling techniques. Fluorophore excitation was provided by means of a low-power fiber-coupled pulsed laser diode operating at 405nm, whose beam had been expanded to a diameter of 2mm before impinging upon the sample. A portion of the sample's isotropic fluorescent emission was collected orthogonal to the incident excitation beam via an analog SiPM and analyzed by waveform sampling at 2.5 GS/s. Fluorophores for system evaluation were selected such that their fluorescence lifetimes differed significantly from one another but their excitation and emission wavelength's maxima did not, and included Coumarin 6, Coumarin 314, and Coumarin 343, each dissolved in ethanol. Measurements were made of a non-fluorescent, isotropicallyscattering specimen to derive the system's response function. Single exponential fitting of the convolution of the system's response function with each dye's intrinsic fluorescence intensity function yielded fluorescence lifetimes in close agreement to those derived from measurements made on a commercial system.
Date:	2016-10-29
Relation:	2016 IEEE Nuclear Science Symposium, Medical Imaging Conference and Room-Temperature Semiconductor Detector Workshop (NSS/MIC/RTSD) 2016 Oct. 29 - Nov. 6;2017 Oct;Article number 8069566.
Link to:	http://dx.doi.org/10.1109/NSSMIC.2016.8069566
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000432419500188
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85041499524
Appears in Collections:	[Leu-Wei Lo] Conference Papers/Meeting Abstract

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