國家衛生研究院 NHRI:Item 3990099045/11057
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    Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/11057


    Title: Laminin & gamma2-rich exosomes enhances lymphangiogenesis via Integrin & alpha3-mediated exosomal uptake
    Other Titles: Laminin & γ2-rich exosomes enhances lymphangiogenesis via Integrin & α3-mediated exosomal uptake
    Authors: Chen, YW;Liu, SH;Liou, GG;Wang, SH;Chang, JS
    Contributors: National Institute of Cancer Research
    Abstract: The oral squamous cell carcinoma sublines, LN1-1 cells have been established by in vivo selection and showed higher abilities of lymphangiogenesis and lymph node metastasis in comparison with their parental OEC-M1 cells. After purified exosomes from cultured medium by differential centrifugation and analyzed using transmitted electron microscopy, dynamic light scattering and Western blot, LN1-1 cell-derived exosomes promoted LEC migration, tube formation and uptake by LECs than OEC-M1 cell-derived exosomes did. Using stable isotope labeling with amino acids in cell culture/liquid chromatography-tandem mass spectrometry based proteomic platform and confirmed by Western blot, Laminin &gamma2 was validated as one of highly expressed proteins in LN1-1-derived exosomes. Knockdown of Laminin &gamma2 impaired migration and tube formation of LECs as well as the exosomal uptake by LECs. Importantly, the silencing of Integrin &alpha3 but not Integrin &beta1, &beta4 and &alpha6 in LECs was able to sustain Laminin &gamma2-mediated exosomal uptake, suggesting a critical role of Integrin &slpha3 in Laminin &gamma2-rich exosome-mediated functions.
    Date: 2018-01
    Relation: Cancer Science. 2018 Jan;109(Suppl. 1):29.
    Link to: http://dx.doi.org/10.1111/cas.13499
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1347-9032&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000422694001055
    Appears in Collections:[Ya-Wen Chen] Conference Papers/Meeting Abstract
    [Jeffrey Shu-Ming] Conference Papers/Meeting Abstract

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