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Please use this identifier to cite or link to this item:
http://ir.nhri.org.tw/handle/3990099045/11101
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| Title: | Stromal C-type lectin receptor COLEC12 integrates H. pylori, PGE2-EP2/4 axis and innate immunity in gastric diseases |
| Authors: | Chang, LL;Hsu, WH;Kao, MC;Chou, CC;Lin, CC;Liu, CJ;Weng, BC;Kuo, FC;Kuo, CH;Lin, MH;Wang, CJ;Lin, CH;Wu, DC;Huang, SK |
| Contributors: | National Institute of Environmental Health Sciences |
| Abstract: | Tissue stroma is known to be important in regulating Hp-mediated inflammation, but its interaction with Hp and dendritic cells (DCs) remains to be determined. To this end, the potential crosstalk between H. pylori (Hp) infected gastric stromal cells (Hp-GSCs) and DCs was investigated. Primary GSCs from cancerous and adjacent normal tissues were generated from gastric cancer patients, and monocyte-derived DCs were obtained from healthy individuals. Levels of cytokines and prostaglandin E2 (PGE2) were measured by ELISA, and C-type lectin expression in GSCs was assessed by flow cytometry and immunohistochemistry. In a trans-well co-culture system, significantly upregulated DC-derived IL-23 expression was found when DCs were co-cultured with Hp-infected GSCs (Hp-GSCs). Further, PGE2 from Hp-GSCs was discovered to possess the priming effect, which could be inhibited by anti-COLEC12 (Collectin subfamily member 12) Abs, COLEC12 knockdown or when alpha3-fucosyltransferase-null (futB; HP0651) strain of Hp was used. Also, the expression of COLEC12 was co-localized with CD90+ stromal cells in cancerous tissues. Hp-GSCs-conditioned DCs were able to induce the expression of IL-17 from CD4+ T cells, which could be inhibited by IL-23-neutralizing Abs. These results suggested the importance of COLEC12 as a receptor involved in Hp-stromal cell interaction and its subsequent conditioning effect on DCs. |
| Date: | 2018-02-28 |
| Relation: | Scientific Reports. 2018 Feb 28;8:Article number 3821. |
| Link to: | http://dx.doi.org/10.1038/s41598-018-20957-2 |
| JIF/Ranking 2023: | http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=2045-2322&DestApp=IC2JCR |
| Cited Times(WOS): | https://www.webofscience.com/wos/woscc/full-record/WOS:000426262400074 |
| Cited Times(Scopus): | http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85042927884 |
| Appears in Collections: | [黃嘯谷] 期刊論文
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| SCP85042927884.pdf | | 1516Kb | Adobe PDF | 271 | View/Open |
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