國家衛生研究院 NHRI:Item 3990099045/11111
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    題名: S- Cis diene conformation: A new bathochromic shift strategy for near-infrared fluorescence switchable dye and the imaging applications
    作者: Chen, HJ;Chew, CY;Chang, EH;Tu, YW;Wei, LY;Wu, BH;Chen, CH;Yang, YT;Huang, SC;Chen, JK;Chen, IC;Tan, KT
    貢獻者: Institute of Biomedical Engineering and Nanomedicine
    摘要: In this paper, we present a novel charge-free fluorescence-switchable near-infrared (IR) dye based on merocyanine for target specific imaging. In contrast to the typical bathochromic shift approach by extending pi-conjugation, the bathochromic shift of our merocyanine dye to the near-IR region is due to an unusual S- cis diene conformer. This is the first example where a fluorescent dye adopts the stable S- cis conformation. In addition to the novel bathochromic shift mechanism, the dye exhibits fluorescence-switchable properties in response to polarity and viscosity. By incorporating a protein-specific ligand to the dye, the probes (for SNAP-tag and hCAII proteins) exhibited dramatic fluorescence increase (up to 300-fold) upon binding with its target protein. The large fluorescence enhancement, near-IR absorption/emission, and charge-free scaffold enabled no-wash and site-specific imaging of target proteins in living cells and in vivo with minimum background fluorescence. We believe that our unconventional approach for a near-IR dye with the S- cis diene conformation can lead to new strategies for the design of near-IR dyes.
    日期: 2018-04-18
    關聯: Journal of the American Chemical Society. 2018 Apr 18;140(15):5224-5234.
    Link to: http://dx.doi.org/10.1021/jacs.8b01159
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0002-7863&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000430642000045
    Cited Times(Scopus): https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85045561647
    顯示於類別:[陳仁焜] 期刊論文

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