國家衛生研究院 NHRI:Item 3990099045/11160

NHRI > National Institute of Infectious Diseases and Vaccinology > Horng-Yunn Dou > Periodical Articles > Item 3990099045/11160

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Title:	An integrated microfluidic system for identification of live mycobacterium tuberculosis by real-time polymerase chain reaction
Authors:	Ip, KU;Chang, JR;Liu, TH;Dou, HY;Lee, GB
Contributors:	National Institute of Infectious Diseases and Vaccinology
Abstract:	Mycobacterium tuberculosis (TB) infection poses a huge challenge worldwide. It is challenging for rapid identification of TB due to its low growth rate, and therefore it could be widely spread. For this reason, a novel combination of propidium monoazide (PMA) and real-time polymerase chain reaction (RT-PCR) was reported in this work for fast detection of live TB. Experimental results showed that the PMA-RT-PCR protocol can detect live TB with a detection limit as low as 14 colony formation units (CFU)/reaction by measuring the threshold cycle number (Ct value) in a calibration curve. Furthermore, PMA could be used successfully to distinguish dead TB such that no PCR products could be generated. More importantly, all the procedure could be finished within 2 hours. This is the first time that an integrated microfluidic system was demonstrated to use molecular diagnostics for fast detection of TB and could be potentially used for prognosis monitoring of TB treatment
Date:	2018-04-26
Relation:	Micro Electro Mechanical Systems (MEMS), 2018 IEEE. 2018 Apr 26;2018-January:1124-1127.
Link to:	http://dx.doi.org/10.1109/MEMSYS.2018.8346758
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000434960900293
Cited Times(Scopus):	https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85047021821
Appears in Collections:	[Horng-Yunn Dou] Periodical Articles

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