國家衛生研究院 NHRI:Item 3990099045/11246
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    題名: Auroramycin: A potent antibiotic from streptomyces roseosporus by CRISPR-Cas9 activation
    作者: Lim, YH;Wong, FT;Yeo, WL;Ching, KC;Lim, YW;Heng, E;Chen, S;Tsai, DJ;Lauderdale, TL;Shia, KS;Ho, YS;Hoon, S;Ang, EL;Zhang, MM;Zhao, H
    貢獻者: Institute of Biotechnology and Pharmaceutical Research;National Institute of Infectious Diseases and Vaccinology
    摘要: Silent biosynthetic gene clusters represent a potentially rich source of new bioactive compounds. We report the discovery, characterization, and biosynthesis of a novel doubly glycosylated 24-membered polyene macrolactam from a silent biosynthetic gene cluster in Streptomyces roseosporus by using the CRISPR-Cas9 gene cluster activation strategy. Structural characterization of this polyketide, named auroramycin, revealed a rare isobutyrylmalonyl extender unit and a unique pair of amino sugars. Relative and absolute stereochemistry were determined by using a combination of spectroscopic analyses, chemical derivatization, and computational analysis. The activated gene cluster for auroramycin production was also verified by transcriptional analyses and gene deletions. Finally, auroramycin exhibited potent anti-methicillin-resistant Staphylococcus aureus (anti-MRSA) activity towards clinical drug-resistant isolates.
    日期: 2018-08
    關聯: Chembiochem: A European Journal of Chemical Biology. 2018 Aug;19(16):1716-1719.
    Link to: http://dx.doi.org/10.1002/cbic.201800266
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1439-4227&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000441744200006
    Cited Times(Scopus): https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85051542619
    顯示於類別:[夏克山] 期刊論文
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