國家衛生研究院 NHRI:Item 3990099045/1125

NHRI > Institute of Biotechnology and Pharmaceutical Research > Shiow-Ju Lee > Periodical Articles > Item 3990099045/1125

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/1125

Title:	Novel small-molecule inhibitors of transmissible gastroenteritis virus
Authors:	Yang, CW;Yang, YN;Liang, PH;Chen, CM;Chen, WL;Chang, HY;Chao, YS;Lee, SJ
Contributors:	Division of Biotechnology and Pharmaceutical Research
Abstract:	We used swine testicle (ST) cells infected with transmissible gastroenteritis virus (TGEV) and an indirect immunofluorescent assay with antibodies against TGEV spike and nucleocapsid proteins to screen small-molecule compounds that inhibit TGEV replication. Analogues of initial hits were collected and subjected to a 3CL protease (3CL(pro)) inhibition assay with recombinant 3CL(pro) and a fluorogenic peptide substrate. A series of benzothiazolium compounds were found to have inhibitory activity against TGEV 3CL pro and to exert anti-TGEV activities in terms of viral protein and RNA replication in TGEV-infected ST cells, with consequent protection of TGEV-infected ST cells from cytopathic effect by blocking the activation of caspase-3.
Keywords:	Microbiology;Pharmacology & Pharmacy
Date:	2007-11
Relation:	Antimicrobial Agents and Chemotherapy. 2007 Nov;51(11):3924-3931.
Link to:	http://dx.doi.org/10.1128/AAC.00408-07
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0066-4804&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000250612900020
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=35848942777
Appears in Collections:	[Shiow-Ju Lee] Periodical Articles [Yu-Sheng Chao(2002-2013)] Periodical Articles

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