國家衛生研究院 NHRI:Item 3990099045/1159
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    题名: High-efficiency protein expression mediated by enterovirus 71 internal ribosome entry
    作者: Lee, JC;Wu, TY;Huang, CF;Yang, FM;Shih, SR;Hsu, JTA
    贡献者: Division of Biotechnology and Pharmaceutical Research
    摘要: : An internal ribosome entry site (IRES) has been used to facilitate the expression of more than one protein in a single transcript. In this study, we examined the translational activities of several IRES elements derived from different RNA viruses. The protein expression of encephalomyocarditis virus (EMCV) IRES is similar to that of hepatitis C virus (HCV)IRES in mammalian cells. Notably, the protein expression of enterovirus 71 (EV71) IRES was 23-fold higher than the efficiency of EMCV IRES following normalization of mRNA transcriptional level. Thus, expression of the secreted alkaline phosphatase (SEAP) reporter protein in mammalian cells may be controlled at desirable levels by using appropriate IRES in the expression vector. (c) 2005 Wiley Periodicals, Inc.
    关键词: Biotechnology & Applied Microbiology
    日期: 2005-06-05
    關聯: Biotechnology and Bioengineering. 2005 Jun;90(5):656-662.
    Link to: http://dx.doi.org/10.1002/bit.20440
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0006-3592&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000229417600014
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=19544364153
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