國家衛生研究院 NHRI:Item 3990099045/11612
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    Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/11612


    Title: Alpha-tubulin acetyltransferase/MEC-17 regulates cancer cell migration and invasion through epithelial-mesenchymal transition suppression and cell polarity disruption
    Other Titles: α-tubulin acetyltransferase/MEC-17 regulates cancer cell migration and invasion through epithelial-mesenchymal transition suppression and cell polarity disruption
    Authors: Lee, CC;Cheng, YC;Chang, CY;Lin, CM;Chang, JY
    Contributors: National Institute of Cancer Research
    Abstract: MEC-17, a newly identified alpha-tubulin-N-acetyltransferase 1, serves as the major alpha-tubulin acetyltransferase to promote alpha-tubulin acetylation in vitro and in vivo. Alteration of alpha-tubulin acetylation may be involved in morphology regulation, cell migration, and tumour metastasis. However, MEC-17's role in cell physiology and its effect on epithelial-mesenchymal transition (EMT) and cell polarity remain elusive. In the present study, we characterized the overexpressed or downregulated cell models through gene targeting as MEC-17 gain- or loss-of-function. Overexpression of MEC-17 enhanced the cell spreading area, suppressed pseudopods formation in a three-dimensional (3D) culture system, and inhibited cancer cell migratory and invasive ability and tumour metastasis by orthotopic lung cancer animal model. Furthermore, morphological change and migration inhibition of cancer cells were accompanied by EMT repression, Golgi reorientation, and polarity disruption caused by alteration of cdc42 activity via a decrease in Rho-GAP, ARHGAP21. By contrast, a reduction in endogenous MEC-17 accelerated the pseudopods formation and EMT, and facilitated cell migration and invasion. These results demonstrated the crucial role of MEC-17 in the modulation of intrinsic cell morphogenesis, migration, and invasive function through regulation of EMT and cell polarity.
    Date: 2018-11
    Relation: Scientific Reports. 2018 Nov;8:Article number 17477.
    Link to: http://dx.doi.org/10.1038/s41598-018-35392-6
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=2045-2322&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000451748100003
    Cited Times(Scopus): https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85057809504
    Appears in Collections:[Jang-Yang Chang] Periodical Articles

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