國家衛生研究院 NHRI:Item 3990099045/12045
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    題名: Genomic analysis of serologically untypable human enteroviruses in Taiwan
    作者: Chien, YS;Luo, ST;Tsao, KC;Huang, YC;Chung, WY;Liao, YC;Tan, Y;Das, SR;Lee, MS
    貢獻者: National Institute of Infectious Diseases and Vaccinology;Institute of Population Health Sciences
    摘要: BackgroundHuman enteroviruses contain over 100 serotypes. We have routinely conducted enterovirus surveillance in northern Taiwan; but about 10% of isolates could not be serotyped using traditional assays. Next-generation sequencing (NGS) is a powerful tool for genome sequencing.MethodsIn this study, we established an NGS platform to conduct genome sequencing for the serologically untypable enterovirus isolates.ResultsAmong 130 serologically untypable isolates, 121 (93%) of them were classified into 29 serotypes using CODEHOP (COnsensus-DEgenerate Hybrid Oligonucleotide Primer)-based RT-PCR to amplify VP1 genes (VP1-CODEHOP). We further selected 52 samples for NGS and identified 59 genome sequences from 51 samples, including 8 samples containing two virus genomes. We also detected 23 genome variants (nucleotide identity <90% compared with genome sequences in the public domain) which were potential genetic recombination, including 9 inter-serotype recombinants and 14 strains with unknown sources of recombination.ConclusionsWe successfully integrated VP1-CODEHOP and NGS techniques to conduct genomic analysis of serologically untypable enteroviruses.
    日期: 2019-07
    關聯: Journal of Biomedical Science. 2019 Jul;26:Article number 49.
    Link to: http://dx.doi.org/10.1186/s12929-019-0541-x
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1021-7770&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000473615400001
    Cited Times(Scopus): https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85068578620
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