國家衛生研究院 NHRI:Item 3990099045/12096

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Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/12096

Title:	Chronic exposure to high fat diet triggers myelin disruption and interleukin-33 upregulation in hypothalamus
Authors:	Huang, HT;Tsai, SF;Wu, HT;Huang, HY;Hsieh, HH;Kuo, YM;Chen, PS;Yang, CS;Tzeng, SF
Contributors:	Institute of Biomedical Engineering and Nanomedicine
Abstract:	BackgroundHypothalamic inflammation including astrogliosis and microglia activation occurs after intake of high fat diet (HFD) in rodent models or in obese individuals. However, the effect of chronic HFD feeding on oligodendrocytes (OLGs), a myelin-producing glial population in the central nervous system (CNS), remains unclear. In this study, we used 8-week old male C57BL/6 mice fed by HFD for 3-6months to induce chronic obesity.ResultsThe transmission electron microscopy imaging analysis showed that the integrity of hypothalamic myelin was disrupted after HFD feeding for 4 and 6months. Moreover, the accumulation of Iba1(+)-microglia with an amoeboid hypertrophic form was continually observed in arcuate nucleus of HFD-fed mice during the entire feeding time period. Interleukin-33 (IL-33), a tissue alarmin upon injury to the CNS, was detected with an increased level in hypothalamus after HFD feeding for 3 and 4months. Furthermore, the in vitro study indicated that exposure of mature OLGs to IL-33 impaired OLG cell structure along with a decline in the expression of myelin basic protein.ConclusionsAltogether, our findings demonstrate that chronic HFD feeding triggers hypothalamic myelin disruption in accompany with IL-33 upregulation and prolonged microglial activation in hypothalamus. Given that the addition of exogenous IL-33 was harmful for the maturation of OLGs, an increase in IL-33 by chronic HFD feeding might contribute to the induction of hypothalamic myelin disruption.
Date:	2019-07
Relation:	BMC Neuroscience. 2019 Jul;20:Article number 33.
Link to:	http://dx.doi.org/10.1186/s12868-019-0516-6
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1471-2202&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000475536600001
Cited Times(Scopus):	https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85068817925
Appears in Collections:	[Chung-Shi Yang] Periodical Articles

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