國家衛生研究院 NHRI:Item 3990099045/12124

NHRI > National Institute of Infectious Diseases and Vaccinology > Shih-Jen Liu > Periodical Articles > Item 3990099045/12124

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/12124

Title:	Domain 4 of pneumolysin from Streptococcus pneumoniae is a multifunctional domain contributing TLR4 activating and hemolytic activity
Authors:	Chiu, FF;Leng, CH;Ding, YJ;Chang, JC;Chang, LS;Lien, SP;Chen, HW;Siu, LK;Liu, SJ
Contributors:	National Institute of Infectious Diseases and Vaccinology
Abstract:	The pneumolysin (Ply) protein of Streptococcus pneumoniae is composed of four domains and possesses several different but related activities. In this study, recombinant Ply and two truncated forms, Ply domain 1-3 and Ply domain 4 (rPly4), were expressed and characterized regarding their participation in apoptosis, the stimulation of cytokine production, hemolytic activity and virulence. rPly4 activated murine bone marrow-derived dendritic cells in a Toll-like receptor (TLR) 4-dependent manner. The rPly4 alone was able to produce hemolytic activity at high concertation and penetrate the lipid bilayer. We further demonstrated that domain 4 of Ply involved in the virulence of the bacteria in mouse model. In the absence of apoptotic activity, the virulence level caused by rPly4 was similar to that of full length Ply. Our data suggested that domain 4 of Ply alone with TLR4 agonist and hemolytic activity may play roles in virulence of Streptococcus pneumoniae.
Date:	2019-10
Relation:	Biochemical and Biophysical Research Communications. 2019 Oct;517(4):596-602.
Link to:	http://dx.doi.org/10.1016/j.bbrc.2019.07.063
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0006-291X&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000506401100008
Cited Times(Scopus):	https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85070094407
Appears in Collections:	[Shih-Jen Liu] Periodical Articles [Leung-Kei Siu] Periodical Articles [Hsin-Wei Chen] Periodical Articles [Chih-Hsiang Leng] Periodical Articles

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