國家衛生研究院 NHRI:Item 3990099045/12211
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    Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/12211


    Title: Rapid single-wavelength lightsheet localization microscopy for clarified tissue
    Authors: Chu, LA;Lu, CH;Yang, SM;Liu, YT;Feng, KL;Tsai, YC;Chang, WK;Wang, WC;Chang, SW;Chen, P;Lee, TK;Hwu, YK;Chiang, AS;Chen, BC
    Contributors: Institute of Molecular and Genomic Medicine
    Abstract: Optical super-resolution microscopy allows nanoscale imaging of protein molecules in intact biological tissues. However, it is still challenging to perform large volume super-resolution imaging for entire animal organs. Here we develop a single-wavelength Bessel lightsheet method, optimized for refractive-index matching with clarified specimens to overcome the aberrations encountered in imaging thick tissues. Using spontaneous blinking fluorophores to label proteins of interest, we resolve the morphology of most, if not all, dopaminergic neurons in the whole adult brain (3.64 x 10(7) microm(3)) of Drosophila melanogaster at the nanometer scale with high imaging speed (436 microm(3) per second) for localization. Quantitative single-molecule localization reveals the subcellular distribution of a monoamine transporter protein in the axons of a single, identified serotonergic Dorsal Paired Medial (DPM) neuron. Large datasets are obtained from imaging one brain per day to provide a robust statistical analysis of these imaging data.
    Date: 2019-10-18
    Relation: Nature Communications. 2019 Oct 18;10:Article number 4762.
    Link to: http://dx.doi.org/10.1038/s41467-019-12715-3
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=2041-1723&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000490982100002
    Cited Times(Scopus): https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85073590920
    Appears in Collections:[Others] Periodical Articles

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