國家衛生研究院 NHRI:Item 3990099045/12292
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    題名: All-optical volumetric physiology for connectomics in dense neuronal structures
    作者: Huang, C;Tai, CY;Yang, KP;Chang, WK;Hsu, KJ;Hsiao, CC;Wu, SC;Lin, YY;Chiang, AS;Chu, SW
    貢獻者: Institute of Molecular and Genomic Medicine
    摘要: All-optical physiology (AOP) manipulates and reports neuronal activities with light, allowing for interrogation of neuronal functional connections with high spatiotemporal resolution. However, contemporary high-speed AOP platforms are limited to single-depth or discrete multi-plane recordings that are not suitable for studying functional connections among densely packed small neurons, such as neurons in Drosophila brains. Here, we constructed a 3D AOP platform by incorporating single-photon point stimulation and two-photon high-speed volumetric recordings with a tunable acoustic gradient-index (TAG) lens. We demonstrated the platform effectiveness by studying the anterior visual pathway (AVP) of Drosophila. We achieved functional observation of spatiotemporal coding and the strengths of calcium-sensitive connections between anterior optic tubercle (AOTU) sub-compartments and >70 tightly assembled 2-mum bulb (BU) microglomeruli in 3D coordinates with a single trial. Our work aids the establishment of in vivo 3D functional connectomes in neuron-dense brain areas.
    日期: 2019-12-20
    關聯: iScience. 2019 Dec 20;22:133-146.
    Link to: http://dx.doi.org/10.1016/j.isci.2019.11.011
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=2589-0042&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000504652000011
    Cited Times(Scopus): https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85075210323
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