| Title: | Quantitative determination of salidroside in rat plasma by on-line solid-phase extraction integrated with high-performance liquid chromatography/electrospray ionization tandem mass spectrometry |
| Authors: | Chang, YW;Yao, HT;Hsieh, SH;Lu, TJ;Yeh, TK |
| Contributors: | Division of Biotechnology and Pharmaceutical Research |
| Abstract: | A method for the quantification of salidroside, a major biologically active compound in Rhodiola, in rat plasma by on-line SPE LC/MS/MS in negative electrospray mode was developed and validated. A column-switching instrument and two HPLC pumping systems were employed, and salicin was used as the internal standard. A Waters Oasis HLB extraction column and an Agilent TC-C-18 analytical column in a column-switching set-up with gradient elution were utilized. The MS/MS ion transitions monitored were m/z 299.0/119.0 and 285.1/122.9 for salidroside and salicin, respectively. The standard curves were linear within a range of 50-5000 ng/mL using weighted linear regression analysis (1/x). The intra- and inter-day coefficients of variance ranged from 1% to 9%. The recovery was above 90%. The freeze/thaw and long-term stability were validated. This method was subsequently applied to a pharmacokinetic study of salidroside in rats. (c) 2007 Elsevier B.V. All rights reserved. |
| Keywords: | Biochemical Research Methods;Chemistry, Analytical |
| Date: | 2007-09-15 |
| Relation: | Journal of Chromatography B. 2007 Sep;857(1):164-169. |
| Link to: | http://dx.doi.org/10.1016/j.jchromb.2007.06.029 |
| JIF/Ranking 2023: | http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1570-0232&DestApp=IC2JCR |
| Cited Times(WOS): | https://www.webofscience.com/wos/woscc/full-record/WOS:000249874400023 |
| Cited Times(Scopus): | http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=34548412679 |
| Appears in Collections: | [Teng-Kuang Yeh] Periodical Articles
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