國家衛生研究院 NHRI:Item 3990099045/12529
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    题名: Development and characterization of standard reagents for cell-based prepandemic influenza vaccine products
    作者: Lai, CC;Weng, TC;Chen, PL;Tseng, YF;Lin, CY;Chia, MY;Sung, WC;Lee, MS;Hu, AY
    贡献者: National Institute of Infectious Diseases and Vaccinology
    摘要: Outbreaks of infection by novel avian influenza virus strains in humans cause public health issues worldwide, and the development of vaccines against such novel strains is the most effective method for the prevention of these virus outbreaks. All types of vaccines must be tested for potency before use; thus, quantitative potency assays are needed for influenza vaccines. The single radial immunodiffusion (SRID) assay is considered the gold standard for quantification of influenza virus antigens, and the SRID reference reagents are essential for the determination of vaccine potency. However, it remains debatable whether reference reagents derived from egg-based vaccine platforms can be used to precisely quantify non-egg-derived vaccines; thus, influenza vaccine production using cell-based platforms has attracted increasing attention. To evaluate the utility of reference reagents derived from a cell-based influenza vaccine platform, we prepared cell-based reference reagents from MDCK cell-grown viruses and compared them with egg-derived reference reagents. A primary liquid standard (PLS) was purified from cell-derived candidate influenza vaccine viruses, and hemagglutinin (HA) antigen content was determined by a densitometric method. The produced PLS could be stored at 4 degrees C for more than 10 months. We also established a simple HA protein purification method for goat antiserum preparation, and the performance of the resulting antiserum was compared to that of standard reagents obtained using different production platforms. The results of this study indicate that these reference reagents can be used for both cell-based and egg-based production platforms and that the differences between these two types of platforms are negligible.
    日期: 2020-09-01
    關聯: Human Vaccines and Immunotherapeutics. 2020 Sep 1;16(9):2245-2251.
    Link to: http://dx.doi.org/10.1080/21645515.2020.1721223
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=2164-5515&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000518279400001
    Cited Times(Scopus): https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85081374828
    显示于类别:[胡勇誌] 期刊論文
    [李敏西] 期刊論文
    [宋旺洲] 期刊論文

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