國家衛生研究院 NHRI:Item 3990099045/12555
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    題名: L-selectin activation regulates Rho GTPase activity via Ca(+2) influx in Sertoli cell line, ASC-17D cells
    作者: Limanjaya, I;Hsu, TI;Chuang, JY;Kao, TJ
    貢獻者: NHRI Graduate Student Program
    摘要: In seminiferous epithelium, tight junctions (TJs) between adjacent Sertoli cells constitute the blood-testis barrier and must change synchronically for germ cells to translocate from the basal to the adluminal compartment during the spermatogenic cycle. Rho GTPase activation through stimulation with specific L-selectin ligands has been proposed to modulate tight junctional dynamics. However, little is known regarding the role of Ca(+2) dynamics in Sertoli cell and how Ca(+2) relays L-selectin signals to modulate Rho GTPase activity in Sertoli cells, thus prompting us to investigate the Ca(+2) flux induced by L-selectin ligand in ASC-17D cells. Using fluorescent real-time image, we first demonstrated the increase of intracellular Ca(+2) level following L-selectin ligand stimulation. This Ca(+2) increase was inhibited in ASC-17D cells pretreated with nifedipine, the L-type voltage-operated Ca(+2) channel (VOCC) blocker, but not mibefradil, the T-type VOCC blocker. We then demonstrated the up-regulation of Rho and Rac1 in ASC-17D cells following the administration of L-selectin ligand, and the pre-treatment with nifedipine, but not mibefradil, prior to L-selectin ligand-binding abolished the activation of both Rho and Rac1. Together, we conclude that the activation of L-selectin induces Ca(+2) influx through the L-type VOCC, which up-regulates Rho and Rac1 proteins, in ASC-17D cells.
    日期: 2020-05
    關聯: Biochemical and Biophysical Research Communications. 2020 May;525(4):1011-1017.
    Link to: http://dx.doi.org/10.1016/j.bbrc.2020.03.011
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0006-291X&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000526804200029
    Cited Times(Scopus): https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85081655883
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