國家衛生研究院 NHRI:Item 3990099045/12599
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    題名: Light energy conversion surface with gold dendritic nanoforests/Si chip for plasmonic polymerase chain reaction
    作者: Huang, HJ;Chiang, YC;Hsu, CH;Chen, JJ;Shiao, MH;Yeh, CC;Huang, SL;Lin, YS
    貢獻者: Institute of Biomedical Engineering and Nanomedicine
    摘要: Surfaces with gold dendritic nanoforests (Au DNFs) on Si chips demonstrate broadband-light absorption. This study is the first to utilize localized surface plasmons of Au DNFs/Si chips for polymerase chain reaction (PCR) applications. A convenient halogen lamp was used as the heating source to illuminate the Au DNFs/Si chip for PCR. A detection target of Salmonella spp. DNA fragments was reproduced in this plasmonic PCR chip system. By semi-quantitation in gel electrophoresis analysis, the plasmonic PCR with 30 cycles and a largely reduced processing time provided results comparable with those of a commercial PCR thermal cycler with 40 cycles in more than 1 h. In the presence of an Au DNFs/Si chip, the plasmonic PCR provides superior results in a short processing time.
    日期: 2020-02-27
    關聯: Sensors. 2020 Feb 27;20(5):Article number 1293.
    Link to: http://dx.doi.org/10.3390/s20051293
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1424-8220&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000525271500055
    Cited Times(Scopus): https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85081031975
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