國家衛生研究院 NHRI:Item 3990099045/12609
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    题名: Microfluidic chip for generating single cell-derived monoclonal cell populations
    作者: Yeh, CF;Lin, CH;Chang, HC;Tang, CY;Lia, PT;Matahum, J;Hsu, CH
    贡献者: Institute of Biomedical Engineering and Nanomedicine
    摘要: In conventional single-cell cloning process, it is difficult to ensure that the obtained cell clones are each populated from a single cell. Therefore several repeats of single-cell cloning are usually required in order to increase the confidence level of obtaining monoclonal cell populations. Here, we present a microfluidic chip which can facilitate the single-cell cloning process by its ability to high-efficiently prepare single cells and populate the cells in a way that their monoclonal status is ensured. We also demonstrated that the chip-generated monoclonal cell populations can be transferred from the chip to a conventional multi-well plate, showing the potential of our chip to become a useful tool for applications where generating monoclonal cells is need.
    日期: 2018-11
    關聯: 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018. 2018 Nov;4:2245-2246.
    Link to: https://cbmsociety.org/microtas/microtas2018/
    Cited Times(Scopus): https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85079758980
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