國家衛生研究院 NHRI:Item 3990099045/12717
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    题名: Promiscuous binding of microprotein mozart1 to gamma-tubulin complex mediates specific subcellular targeting to control microtubule array formation
    其它题名: Promiscuous binding of microprotein mozart1 to γ-tubulin complex mediates specific subcellular targeting to control microtubule array formation
    作者: Huang, TL;Wang, HJ;Chang, YC;Wang, SW;Hsia, KC
    贡献者: Institute of Molecular and Genomic Medicine
    摘要: How γ-tubulin ring complex (γ-TuRC), a master template for microtubule nucleation, is spatially and temporally regulated for the assembly of new microtubule arrays remains unclear. Here, we report that an evolutionarily conserved microprotein, Mozart1 (Mzt1), regulates subcellular targeting and microtubule formation activity of γ-TuRC at different cell cycle stages. Crystal structures of protein complexes demonstrate that Mzt1 promiscuously interacts with the N-terminal domains of multiple γ-tubulin complex protein subunits in γ-TuRC via an intercalative binding mode. Genetic- and microscopy-based analyses show that promiscuous binding of Mzt1 in γ-TuRC controls specific subcellular localization of γ-TuRC to modulate microtubule nucleation and stabilization in fission yeast. Moreover, we find Mzt1-independent targeting of γ-TuRC to be crucial for mitotic spindle assembly, demonstrating the cell-cycle-dependent regulation and function of γ-TuRC. Our findings reveal a microprotein-mediated regulatory mechanism underlying microtubule cytoskeleton formation, whereby Mzt1 binding promiscuity confers localization specificity on the multi-protein complex γ-TuRC.
    日期: 2020-06-30
    關聯: Cell Reports. 2020 Jun 30;31(13):Article number 107836.
    Link to: http://dx.doi.org/10.1016/j.celrep.2020.107836
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=2211-1247&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000546045900021
    Cited Times(Scopus): https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85087022671
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