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http://ir.nhri.org.tw/handle/3990099045/12722
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| Title: | lncRNA UBA6-AS1 participates in the integrated stress response of breast cancer |
| Authors: | Wu, YZ;Chen, YH;Cheng, CT;Chi, KV;Kuo, TC;Kung, HJ;Ann, DK;Kuo, CY |
| Contributors: | Institute of Molecular and Genomic Medicine |
| Abstract: | lncRNA, UBA6-AS1, was identified to be upregulated to promote breast cancer cell death upon glucose deprivation. Besides of glucose deprivation, UBA6-AS1 was also induced by the deprivation of amino acids including glutamine and arginine in several breast cancer cell lines, suggesting that the upregulation of UBA6-AS1 was a universal metabolic stress event. We also found that UBA6-AS1 expression was increased upon the administration of ER stress inducers, tunicamycin (Tm) and thapsigargin (Tg) in breast cancer cells, implicating a potential role of UBA6-AS1 in harmonizing the nutrient and ER stresses. Moreover, after analyzing the genomic position and sequence of UBA6-AS1, activating transcription factor 4 (ATF4), a critical regulator in the ISR coordinating nutrient and ER stress signaling for controlling cell survival and stress adaption, has been predicted to be the regulator of UBA6-AS1 upon the induction of nutrient stress, further supporting the role of UBA6-AS1 participating in the ISR of breast cancer cells. Depletion of UBA6-AS1 rendered breast cancer cells more resistant to nutrient deprivation, and the opposite results were observed when UBA6-AS1 was overexpressed, indicating that UBA6-AS1 may participate in the regulation of breast cancer cell survival under metabolic stress. To investigate the function of UBA6-AS1, RNA-sequencing (RNA-seq) was performed to profile gene expression in breast cancer cells overexpressing UBA6-AS1. The RNA-seq analysis revealed that the top 10 enriched biological processes were mostly related to apoptosis or programmed cell death in the UBA6-AS1 overexpressing cells, suggesting that the up-regulation of UBA6-AS1 may induce apoptosis in response to metabolic stress. In the future, we will focus on the molecular mechanism of the regulation and function of UBA6-AS1 as well as its biological role and association with breast cancer progression. |
| Date: | 2020-02 |
| Relation: | Cancer Research. 2020 Feb;80(4, Suppl.):Meeting Abstract P1-05-10. |
| Link to: | http://dx.doi.org/10.1158/1538-7445.Sabcs19-p1-05-10 |
| JIF/Ranking 2023: | http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0008-5472&DestApp=IC2JCR |
| Cited Times(WOS): | https://www.webofscience.com/wos/woscc/full-record/WOS:000527012500210 |
| Appears in Collections: | [龔行健] 期刊論文
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