國家衛生研究院 NHRI:Item 3990099045/12804
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    题名: Aptamer probed isolation of circulating tumor cells in cholangiocarcinoma patients
    作者: Gopinathan, P;Chiang, NJ;Wang, CH;Sinha, A;Tsai, YC;Tu, HC;Hung, SC;Hsu, PH;Shan, YS;Lee, GB
    贡献者: National Institute of Cancer Research
    摘要: Cholangiocarcinoma (CCA) is a typically lethal, highly metastatic cancer which poses diagnostic challenges and is consequently associated with poor prognosis and high metastatic capability. Since the currently used diagnostic biomarkers are limited in sensitivity and specificity, probes with high-affinity and good specificity are needed to study metastasis in CCA. Circulating tumor cells (CTCs) in blood have been widely accepted as an indicator of diseases progression and metastasis in various other cancers. We hereby report the first use of a CCA specific aptamer probe, coupled on magnetic beads for the isolation of CCA CTCs from 35 advanced stage/metastatic patients on an integrated microfluidic platform with 100 % efficiency (>1 CTC/mL were isolated). The isolated cells were identified via immunofluorescence staining (CK+CD45−Hoechst+) and cell counting was assisted by ImageJ. The binding target of the aptamer on the CCA cancer cells was further analyzed and determined to be desmoplakin by liquid chromatography-mass spectrometry, western blotting, and immunofluorescence staining. It is envisioned that this biosensing platform could therefore be used for proactive CCA diagnostics in the near future.
    日期: 2020-11-01
    關聯: Sensors and Actuators B: Chemical. 2020 Nov 1;322:Article number 128569.
    Link to: http://dx.doi.org/10.1016/j.snb.2020.128569
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0925-4005&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000573296500013
    Cited Times(Scopus): https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85087808583
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