國家衛生研究院 NHRI:Item 3990099045/1292
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    題名: Anti-sense expression of a metallopeptidase gene enhances nuclear entry of HBV-DNA
    作者: Yeh, CT;Lai, HY;Chu, SP;Tseng, IC
    貢獻者: Division of Biotechnology and Pharmaceutical Research
    摘要: Although several putative hepatitis B virus (HBV) receptors have been identified, none of them is capable of initiating HBV replication in a non-permissive human cell line. Using an Epstein-Barr virus-based extrachromosomal replication system, we have screened through a human liver cDNA library and successfully identified a clone capable of facilitating nuclear transport of HBV-DNA during the early phase of HBV infection. This clone contained a cDNA encoding a metallopeptidase-like protein in anti-sense orientation. Pretreatment of naive HepG2 cells with 1,10-phenanthroline, an inhibitor for liver metallopeptidases, led to nuclear entry of HBV-DNA after HBV infection. However, cccDNA was still undetectable in the nuclei, indicating other cellular factors required to complete the replication cycle were still missing. Our present data suggest that in the initial stage of HBV infection, liver metalopeptidase constitutes a barrier for effective nuclear entry of HBV genomic DNA. Attenuation of metallopeptidase activity may facilitate HBV infection. (C) 2004 Elsevier Inc. All rights reserved.
    關鍵詞: Biochemistry & Molecular Biology;Biophysics
    日期: 2004-10-08
    關聯: Biochemical and Biophysical Research Communications. 2004 Oct;323(1):32-37.
    Link to: http://dx.doi.org/10.1016/j.bbrc.2004.08.079
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0006-291X&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000223965300005
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=4444382529
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