國家衛生研究院 NHRI:Item 3990099045/13175
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    题名: The prevention and augmentation of tetravalent recombinant measles-vectored dengue vaccine in dengue-2 infected AG129 mice
    作者: Pan, CH;Lin, TH;Yan, JY
    贡献者: National Institute of Infectious Diseases and Vaccinology
    摘要: Dengue is an emerging mosquito-borne disease and the use of prophylactic vaccine is still limited. We have previously developed the tetravalent dengue vaccine (rMV-TDV) by a recombinant measles virus (MV) vector expressing envelope protein domain III (ED3). In this study, we used AG129 mice to evaluate the protective and/or pathogenic immune responses induced by rMV-TDV. Immunization of rMV-TDV has induced a significant neutralizing antibody response against all serotypes of DENV, as well as a significant CD4-dependent IFN-γ response biased to DENV-3, compared to the vector controls. After DENV-2 challenge, rMV-TDV-immunized mice showed a significant lower viremia and no increase of inflammatory cytokines comparing to the vector controls, which had a ~100 times higher viremia and a significant increase of IFN-γ and TNF-α 3 days after challenge. A robust DENV-2 specific IFN-γ response detected in rMV-TDV-immunized mice suggested that pre-existing DENV-3 biased T-cell responses didn’t cross-react to DENV-2 but an anamnestic DENV-2 specific T-cell response was recalled. Prior to the challenge, only DENV-3 specific IgG demonstrated a significant higher avidity than other serotypes, whose specific IgG avidity increased after DENV-2 challenge, particular in DENV-1 and -2. However, the passive transfer of rMV-TDV immunized sera plus splenocytes resulted in the higher viremia and the significant increase of inflammatory cytokines in AG129 recipient mice after DENV-2 challenge than the vector control donors did. It suggested that while the immunity by rMV-TDV waned, the potential risks for the severe disease increased. Our data shed the lights on the role of a balanced CD4 T-cell response in the prevention of severe dengue.
    日期: 2020-05
    關聯: Journal of Immunology. 2020 May;204(1, Suppl.):Meeting Abstract 167.24.
    Link to: https://www.jimmunol.org/content/204/1_Supplement/167.24
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0022-1767&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000589972402080
    显示于类别:[潘建雄] 會議論文/會議摘要

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