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Please use this identifier to cite or link to this item:
http://ir.nhri.org.tw/handle/3990099045/13176
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| Title: | The use of maldi-tof mass spectrometry to analyze commensal oral yeasts in nursing home residents |
| Authors: | Lu, JJ;Lo, HJ;Lee, CH;Chen, MJ;Lin, CC;Chen, YZ;Tsai, MH;Wang, SH |
| Contributors: | National Institute of Infectious Diseases and Vaccinology |
| Abstract: | Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a rapid and accurate method to identify microorganisms in clinical laboratories. This study isolates yeast-like microorganisms in the oral washes that are collected from non-bedridden nursing home residents, using CHROMagar Candida plates, and identifies them using Bruker MALDI-TOF MS. The ribosomal DNA sequences of the isolates are then examined. Three hundred and twenty yeast isolates are isolated from the oral washes. Candida species form the majority (78.1%), followed by Trichosporon/Cutaneotrichosporon species (8.8%). Bruker MALDI-TOF MS gives a high-level confidence, with a log(score) value of ≥1.8, and identifies 96.9% of the isolates. There are six inconclusive results (1.9%), and those sequences are verified as rare clinical species, including Candida ethanolica, Cutaneotrichosporon jirovecii, Exophiala dermatitidis, and Fereydounia khargensis. Almost all of the isolates have a regular color on the CHROMagar Candida plates. If the colonies are grouped by color on the plates, a specific dominant yeast species is present in each color group, except for purple or orange isolates. In conclusion, MALDI-TOF MS is verified as a fast, accurate and practical method to analyze oral yeasts in elderly subjects. |
| Date: | 2021-01-09 |
| Relation: | Microorganisms. 2021 Jan 9;9(1):Article number 142. |
| Link to: | http://dx.doi.org/10.3390/microorganisms9010142 |
| JIF/Ranking 2023: | http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=2076-2607&DestApp=IC2JCR |
| Cited Times(WOS): | https://www.webofscience.com/wos/woscc/full-record/WOS:000610589100001 |
| Cited Times(Scopus): | https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85099244292 |
| Appears in Collections: | [羅秀容] 期刊論文
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| SCP85099244292.pdf | | 3644Kb | Adobe PDF | 265 | View/Open |
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