國家衛生研究院 NHRI:Item 3990099045/13423
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    題名: Generating mutant Aedes aegypti mosquitoes using the CRISPR/Cas9 system
    作者: Li, HH;Li, JC;Su, MP;Liu, KL;Chen, CH
    貢獻者: National Institute of Infectious Diseases and Vaccinology;National Mosquito-Borne Diseases Control Research Center
    摘要: Implementation of CRISPR/Cas9 methodologies for mosquito gene editing has not yet become widespread. This protocol details the procedure for Aedes aegypti mosquito gene editing using homology-directed repair and fluorescent marker insertion, which facilitates the generation and screening of mutant mosquito lines for gene function testing. We describe optimized methods for single guide RNA plasmid preparation, homologous recombination donor plasmid construction, embryo microinjection, and precise gene knock-in confirmation. We also provide general guidance for establishing mutant mosquito lines. For details on the practical use and execution of this protocol, please refer to Li et al. (2020).
    日期: 2021-06-18
    關聯: STAR Protocols. 2021 Jun 18;2(2):Article number 100432.
    Link to: http://dx.doi.org/10.1016/j.xpro.2021.100432
    Cited Times(Scopus): https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85103778868
    顯示於類別:[陳俊宏] 期刊論文
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