國家衛生研究院 NHRI:Item 3990099045/13601

NHRI > Institute of Biomedical Engineering and Nanomedicine > Guo-Chung Dong > Periodical Articles > Item 3990099045/13601

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/13601

Title:	A cyclic BMP-2 peptide upregulates BMP-2 protein-induced cell signaling in myogenic cells
Authors:	Gudivada, VN;Huang, CJ;Luo, YH;Dong, GC
Contributors:	Institute of Biomedical Engineering and Nanomedicine
Abstract:	In the current study, we designed four cyclic peptide analogues by incorporating two cysteine residues in a BMP-2 linear knuckle epitope in such a way that the active region of the peptide could be either inside or outside the cyclic ring. Bone morphogenetic protein receptor BMPRII was immobilized on the chip surface, and the interaction of the linear and cyclic peptide analogues was studied using surface plasmon resonance (SPR). From the affinity data, the peptides with an active region inside the cyclic ring had a higher binding affinity in comparison to the other peptides. To confirm that our affinity data are in line in vitro, we studied the expression levels of RUNX2 (runt-related transcription factor) and conducted an osteogenic marker alkaline phosphatase (ALP) assay and staining. Based on the affinity data and the in vitro experiments, peptide P-05 could be a suitable candidate for osteogenesis, with higher binding affinity and increased RUNX2 and ALP expression in comparison to the linear peptides
Date:	2021-07-31
Relation:	Polymers. 2021 Jul 31;13(15):Article number 2549.
Link to:	https://doi.org/10.3390/polym13152549
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=2073-4360&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000682197000001
Cited Times(Scopus):	https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85112107570
Appears in Collections:	[Guo-Chung Dong] Periodical Articles

Files in This Item:

File	Description	Size	Format
NBN2021080501.pdf		4418Kb	Adobe PDF	275	View/Open

Loading...