國家衛生研究院 NHRI:Item 3990099045/13627
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    题名: Exfoliated tumor cells in bile as a promising indicator of disease status in cholangiocarcinoma
    作者: Gopinathan, P;Lu, TH;Chiang, NJ;Huang, CJ;Tu, HC;Hung, SC;Shan, YS;Lee, GB
    贡献者: National Institute of Cancer Research
    摘要: Cholangiocarcinoma (CCA) is highly metastatic, extremely difficult to diagnose and is consequently characterized by a low 5-year survival rate. Herein we investigated the potential of using exfoliated tumor cells (ETCs) in bile to serve as an early-warning CCA metastasis indicator. ETCs were isolated from bile using a CCA-specific aptamer conjugated to magnetic beads and identified via immunostaining (CK17+CK7+Hoechst+). A conventional protein biomarker, epithelial cell adhesion molecule (EpCAM), was used for comparison. Circulating tumor cell (CTC) isolation in blood was achieved by a microfluidic system from samples from five CCA patients undergoing chemotherapy, and the enumeration results of ETCs and CTCs were compared with diagnoses derived from computed tomography scans. The CCA-specific aptamer-conjugated magnetic beads effectively bound ETCs from bile samples of 40/40 CCA patients (>1 ETC in 3 ml of bile), and the ETC and CTC count data from patients corresponded well with cancer progression and tumor size. ETCs in bile could serve as promising indicators of disease status in early-advanced stage CCA while CTCs in blood could be representative of late-advanced stage CCA. The developed technique could consequently be a powerful tool in CCA diagnostics and prognostics.
    日期: 2021-11-01
    關聯: Sensors and Actuators B: Chemical. 2021 Nov 01;346:Article number 130526.
    Link to: http://dx.doi.org/10.1016/j.snb.2021.130526
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0925-4005&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000691811000001
    Cited Times(Scopus): https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85111635502
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