國家衛生研究院 NHRI:Item 3990099045/13648

NHRI > National Institute of Infectious Diseases and Vaccinology > Horng-Yunn Dou > Periodical Articles > Item 3990099045/13648

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/13648

Title:	Characterization of virus replication, pathogenesis, and cytokine responses in syrian hamsters inoculated with SARS-CoV-2
Authors:	Yang, SJ;Wei, TC;Hsu, CH;Ho, SN;Lai, CY;Huang, SF;Chen, YY;Liu, SJ;Yu, GY;Dou, HY
Contributors:	National Institute of Infectious Diseases and Vaccinology;Institute of Molecular and Genomic Medicine;Pathology Core Laboratory
Abstract:	BACKGROUND: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a novel coronavirus which caused a global respiratory disease pandemic beginning in December 2019. Understanding the pathogenesis of infection and the immune responses in a SARS-CoV-2-infected animal model is urgently needed for vaccine development. METHODS: Syrian hamsters (Mesocricetus auratus) were intranasally inoculated with 10(5), 5×10(5), and 10(6) TCID(50) of SARS-CoV-2 per animal and studied for up to 14 days. Body weight, viral load and real-time PCR amplification of the SARS-CoV-2 N gene were measured. On days 3, 6 and 9, lung, blood, liver, pancreas, heart, kidney, and bone marrow were harvested and processed for pathology, viral load, and cytokine expression. RESULTS: Body weight loss, increased viral load, immune cell infiltration, upregulated cytokine expression, viral RNA, SARS-CoV-2 nucleoprotein, and mucus were detected in the lungs, particularly on day 3 post-infection. Extremely high expression of the pro-inflammatory cytokines MIP-1 and RANTES was detected in lung tissue, as was high expression of IL-1β, IL-6, IL-12, and PD-L1. The glutamic oxalacetic transaminase/glutamic pyruvic transaminase (GOT/GPT) ratio in blood was significantly increased at 6 days post-infection, and plasma amylase and lipase levels were also elevated in infected hamsters. CONCLUSION: Our results provide new information on immunological cytokines and biological parameters related to the pathogenesis and immune response profile in the Syrian hamster model of SARS-CoV-2 infection.
Date:	2021-08-11
Relation:	Journal of Inflammation Research. 2021 Aug 11;14:3781-3795.
Link to:	http://dx.doi.org/10.2147/jir.S323026
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1178-7031&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000757948500001
Cited Times(Scopus):	https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85113137827
Appears in Collections:	[Horng-Yunn Dou] Periodical Articles [Guann-Yi Yu] Periodical Articles [Shih-Jen Liu] Periodical Articles [Shiu-Feng Kathy Huang] Periodical Articles [其他] Periodical Articles

Files in This Item:

File	Description	Size	Format
PUB34408462.pdf		10737Kb	Adobe PDF	167	View/Open

Loading...