國家衛生研究院 NHRI:Item 3990099045/13681
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    題名: KFP-1, a novel calcium-binding peptide isolated from kefir, promotes calcium influx through TRPV6 channels
    作者: Chang, GR;Tu, MY;Chen, YH;Chang, KY;Chen, CF;Lai, JC;Tung, YT;Chen, HL;Fan, HC;Chen, CM
    貢獻者: NHRI Graduate Student Program
    摘要: SCOPE: Kefir is an acidic and alcoholic fermented milk product with multiple health-promoting benefits. Our previous study demonstrated that kefir enhanced calcium absorption in intestinal Caco-2 cells. In this study, kefir-fermented peptide-1 (KFP-1) was isolated from the kefir peptide fraction, and its function as a calcium-binding peptide was characterized. METHODS AND RESULTS: KFP-1 was identified as a 17-residue peptide with a sequence identical to that of κ-casein (residues 138-154) in milk protein. KFP-1 was demonstrated to promote calcium influx in Caco-2 and IEC-6 small intestinal cells in a concentration-dependent manner. TRPV6, but not L-type voltage-gated calcium channels, was associated with the calcium influx induced by KFP-1. An in vitro calcium binding assay indicated that the full-length KFP-1 peptide has a higher calcium-binding capacity than the two truncated KFP-1 peptides, KFP-1∆C5 and KFP-1C5. Alexa Fluor 594 labeling showed that KFP-1 is taken up by Caco-2 cells and interacts with calcium ions and TRPV6 protein. Moreover, KFP-1 was found moderately resistant to pepsin and pancreatin digestions and enhanced calcium uptake by intestinal enterocytes in vivo. CONCLUSION: These data suggest that KFP-1, a novel calcium-binding peptide, binds extracellular calcium ions and enters Caco-2 and IEC-6 cells, and promotes calcium uptake through TRPV6 calcium channels. The present study is of great importance for developing kefir-derived metal ion-binding peptides as functional nutraceutical additives.
    日期: 2021-11
    關聯: Molecular Nutrition and Food Research. 2021 Nov;65(22):Article number e2100182.
    Link to: http://dx.doi.org/10.1002/mnfr.202100182
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1613-4125&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000708747700001
    Cited Times(Scopus): https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85117201544
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