國家衛生研究院 NHRI:Item 3990099045/14040
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    题名: Investigating a boronate-affinity-guided acylation reaction for labelling native antibodies
    作者: Adak, AK;Huang, KT;Liao, CY;Lee, YJ;Kuo, WH;Huo, YR;Li, PJ;Chen, YJ;Chen, BS;Chen, YJ;Chu Hwang, K;Wayne Chang, WS;Lin, CC
    贡献者: National Institute of Cancer Research
    摘要: The excellent molecular recognition capabilities of monoclonal antibodies (mAbs) have opened up exciting opportunities for biotherapeutic discovery. Taking advantage of the full potential of this tool necessitates affinity ligands capable of conjugating directly with small molecules to a defined degree of biorthogonality, especially when modifying natural Abs. Herein, a bioorthogonal boronate-affinity-based Ab ligand featuring a 4-(dimethylamino)pyridine and an S-aryl thioester to label full-length Abs is reported. The photoactivatable linker in the acyl donor facilitated purification of azide-labelled Ab (N3-Ab) was quantitatively cleaved upon brief exposure to UV light while retaining the original Ab activity. Click reactions enabled the precise addition of biotin, a fluorophore, and a pharmacological agent to the purified N3-Abs. The resulting immunoconjugate showed selectivity against targeted cells. Bioorthogonal traceless design and reagentless purification allow this strategy to be a powerful tool to engineer native antibodies amenable to therapeutic intervention.
    日期: 2022-03-22
    關聯: Chemistry - A European Journal. 2022 Mar 22;28(17):Article number e202104178.
    Link to: http://dx.doi.org/10.1002/chem.202104178
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0947-6539&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000757858800001
    Cited Times(Scopus): https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85124827809
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