國家衛生研究院 NHRI:Item 3990099045/1405
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    Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/1405


    Title: Synergism of biochemical and mechanical stimuli in the differentiation of human placenta-derived multipotent cells into endothelial cells
    Authors: Wu, CC;Chao, YC;Chen, CN;Chien, S;Chen, YC;Chien, CC;Chiu, JJ;Yen, BL
    Contributors: Stem Cell Research Center;Division of Medical Engineering Research
    Abstract: There have been intensive studies on the differentiation of endothelial progenitor cells (EPCs) into endothelial cells. We investigated the endothelial differentiation of placenta-derived multipotent cells (PDMCs), a population of CD34(-)/CD133(-)/FIk-1(-) cells. PDMCs were cultured in basal media or media containing endothelial growth factors (EGM), including vascular endothelial growth factor (VEGF), for 3 days and then subjected to shear stress of 6 or 12 dyn/cm(2) for 24 h. Culture of PDMCs in EGM under static conditions resulted in significant increases in VEGF receptor-1 (Flt-1) and receptor-2 (Flk-1) expression. Application of shear stress at 12 dyn/cm(2) to these cells led to significant increases in their expression of von Willebrand Factor and platelet-endothelial cell adhesion molecule-1 at both the gene and protein levels. Shear stress at 6 dyn/cm(2) had lesser effects. Uptakes of acetylated low-density lipoproteins as well as formation of tube-like structures on Matrigel were significantly increased after subjecting to shear stress of 12dyn/cm(2) for 24 h. Our findings suggest that the combined use of endothelial growth factors and high shear stress is synergistic for the endothelial differentiation of PDMCs. (C) 2007 Elsevier Ltd. All rights reserved.
    Keywords: Biophysics;Engineering, Biomedical
    Date: 2008
    Relation: Journal of Biomechanics. 2008;41(4):813-821.
    Link to: http://dx.doi.org/10.1016/j.jbiomech.2007.11.008
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0021-9290&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000254677400011
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=39749100044
    Appears in Collections:[Betty Lin-Ju Yen] Periodical Articles
    [Jeng-Jiann Chiu] Periodical Articles
    [Yao-Chang Chen(2002-2004)] Periodical Articles

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