國家衛生研究院 NHRI:Item 3990099045/14188
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    題名: A portable controllable compressive stress device to monitor human breast cancer cell protrusions at single-cell resolution
    作者: Yeh, CF;Juang, DS;Chen, YW;Rodoplu, D;Hsu, CH
    貢獻者: Institute of Biomedical Engineering and Nanomedicine;National Institute of Cancer Research
    摘要: In vitro devices offer more numerous methods than in vivo models to investigate how cells respond to pressure stress and quantify those responses. Several in vitro devices have been developed to study the cell response to compression force. However, they are unable to observe morphological changes of cells in real-time. There is also a concern about cell damage during the process of harvesting cells from 3D gels. Here we report a device employing transparent, thin gel layers to clamp cells between the interfaces and applied a controllable compression force by stacking multiple layers on the top. In this approach, cells can be monitored for alteration of cellular protrusions, whose diversity has been proven to promote cancer cell dissemination, with single-cell resolution under compression force. Furthermore, p-Rac-1 and rhodamine staining on the device directly to confirm the actin filaments of lamellipodia. The method was able to fulfill real-time live-cell observation at single-cell resolution and can be readily used for versatile cell analysis. MDA-MB-231 and MCF7 breast cancer cells were utilized to demonstrate the utility of the device, and the results showed that the stimuli of compression force induce MDA-MB-231 and MCF7 to form lamellipodia and bleb protrusions, respectively. We envision the device may be used as a tool to explore mechanisms of membrane protrusion transitions and to screen drug candidates for inhibiting cancer cell protrusion plasticity for cancer therapy.
    日期: 2022-02
    關聯: Frontiers in Bioengineering and Biotechnology. 2022 Feb;10:Article number 852318.
    Link to: http://dx.doi.org/10.3389/fbioe.2022.852318
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=2296-4185&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000769357000001
    Cited Times(Scopus): https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85126190842
    顯示於類別:[許佳賢] 期刊論文
    [陳雅雯] 期刊論文

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