Chronic cutaneous wounds from tissue trauma or extensive burns could impair skin barrier function and cause severe infection. Fabrication of a customizable tissue-engineered skin is a promising strategy for regeneration of uneven wounds. Herein, we developed a planar-/curvilinear-bioprintable hydrogel to produce tissue-engineered skin and evaluated in rat models of chronic and irregular wounds. The hydrogel was composed of biodegradable polyurethane (PU) and gelatin. The hydrogel laden with cells displayed good three-dimensional printability and structure stability. The circular wounds of normal and diabetes mellitus (DM) rats treated with planar-printed tri-cell-laden (fibroblasts, keratinocytes, and endothelial progenitor cells) hydrogel demonstrated full re-epithelization and dermal repair as well as large amounts of neovascularization and collagen production after 28 days. Furthermore, the curvilinear module was fabricated based on the corresponding wound topography for curvilinear-bioprinting of the irregular tissue-engineered skin. The large and irregular rat skin wounds treated with curvilinear-printed tri-cell-laden hydrogel demonstrated full repair after 28 days. This planar-/curvilinear-bioprintable tri-cell-laden hydrogel shows great potential for customized biofabrication in skin tissue engineering. This article is protected by copyright. All rights reserved.
Date:
2022-08-17
Relation:
Advanced Healthcare Materials. 2022 Aug 17;Article number e2201021.
