國家衛生研究院 NHRI:Item 3990099045/14341

NHRI > National Institute of Infectious Diseases and Vaccinology > Shih-Jen Liu > Periodical Articles > Item 3990099045/14341

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/14341

Title:	A DNA vaccine candidate delivered by an electroacupuncture machine provides protective immunity against SARS-CoV-2 infection
Authors:	Tzeng, TT;Chai, KM;Shen, KY;Yu, CY;Yang, SJ;Huang, WC;Liao, HC;Chiu, FF;Dou, HY;Liao, CL;Chen, HW;Liu, SJ
Contributors:	National Institute of Infectious Diseases and Vaccinology
Abstract:	A major challenge in the use of DNA vaccines is efficient DNA delivery in vivo. Establishing a safe and efficient electric transfer method is the key to developing rapid DNA vaccines against emerging infectious diseases. To overcome the complexity of designing new electric transfer machines for DNA delivery, a clinically approved electric transfer machine could be considered as an alternative. Here, we report an electroacupuncture machine-based method for DNA vaccine delivery after intramuscular injection of the COVID-19 DNA vaccine. The S gene of SARS-CoV-2 in the pVAX1 plasmid (pSARS2-S) was used as an antigen in this study. We optimized the clinically used electroacupuncture machine settings for efficient induction of the neutralizing antibody titer after intramuscular injection of pSARS2-S in mice. We found that pSARS2-S immunization at 40 Vpp for 3-5 s could induce high neutralizing antibody titers and Th1-biased immune responses. IFN-gamma/TNF-alpha-secreting CD4(+) and CD8(+) T cells were also observed in the DNA vaccination group but not in the recombinant protein vaccination group. T-cell epitope mapping shows that the major reactive epitopes were located in the N-terminal domain (a.a. 261-285) and receptor-binding domain (a.a. 352-363). Importantly, pSARS2-S immunization in hamsters could induce protective immunity against SARS-CoV-2 challenge in vivo. In the preclinical toxicology study, blood biochemistry, hematology, and DNA persistence analysis reveal that the DNA delivery method is safe. Furthermore, the raised antisera could also cross-neutralize different variants of concern. These findings suggest that DNA vaccination using an electroacupuncture machine is feasible for use in humans in the future.
Date:	2022-06-03
Relation:	npj Vaccines. 2022 Jun 03;7:Article number 60.
Link to:	http://dx.doi.org/10.1038/s41541-022-00482-0
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=2059-0105&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000805787600001
Cited Times(Scopus):	https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85131206486
Appears in Collections:	[Shih-Jen Liu] Periodical Articles [Hsin-Wei Chen] Periodical Articles [Ching-Len Liao] Periodical Articles [Horng-Yunn Dou] Periodical Articles [Chia-Yi Yu] Periodical Articles [Tsai-Teng Tzeng] Periodical Articles

Files in This Item:

File	Description	Size	Format
ISI000805787600001.pdf		3761Kb	Adobe PDF	173	View/Open

Loading...