國家衛生研究院 NHRI:Item 3990099045/14535

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Title:	Distribution and genomic characterization of third-generation cephalosporin-resistant escherichia coli Isolated from a single family and home environment: A 2-year longitudinal study
Authors:	Feng, YC;Liou, CH;Ng, WV;Chen, FJ;Hung, CH;Liu, PY;Liao, YC;Wu, HC;Cheng, MF
Contributors:	National Institute of Infectious Diseases and Vaccinology;Institute of Population Health Sciences
Abstract:	Third-generation cephalosporin-resistant Escherichia coli (CREC), particularly strains producing extended-spectrum β-lactamases (ESBLs), are a global concern. Our study aims to longitudinally assemble the genomic characteristics of CREC isolates from fecal samples from an index patient with recurrent CREC-related urinary tract infections and his family and swabs from his home environment 12 times between 2019 and 2021 to investigate the distribution of antibiotic resistance genes. CREC identified using the VITEK 2 were subjected to nanopore whole-genome sequencing (WGS). The WGS of 27 CREC isolates discovered in 137 specimens (1 urine, 123 feces, and 13 environmental) revealed the predominance of ST101 and ST131. Among these sequence types, bla(CTX-M) (44.4%, n = 12) was the predominant ESBL gene family, with bla(CTX-M-14) (n = 6) being the most common. The remaining 15 (55.6%) isolates harbored bla(CMY-2) genes and were clonally diverse. All E. coli isolated from the index patient's initial urine and fecal samples belonged to O25b:H4-B2-ST131 and carried bla(CTX-M-14). The results of sequence analysis indicate plasmid-mediated household transmission of bla(CMY-2) or bla(CTX-M-55). A strong genomic similarity was discovered between fecal ESBL-producing E. coli and uropathogenic strains. Furthermore, bla(CMY-2) genes were widely distributed among the CREC isolated from family members and their home environment.
Date:	2022-08-25
Relation:	Antibiotics. 2022 Aug 25;11(9):Article number 1152.
Link to:	http://dx.doi.org/10.3390/antibiotics11091152
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=2079-6382&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000857618000001
Cited Times(Scopus):	https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85138531739
Appears in Collections:	[Feng-Jui Chen] Periodical Articles [Yu-Chieh Liao] Periodical Articles

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