國家衛生研究院 NHRI:Item 3990099045/1453

NHRI > Institute of Biomedical Engineering and Nanomedicine > Kurt Ming-Chao Lin > Periodical Articles > Item 3990099045/1453

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/1453

Title:	Myocyte protection by 10 kD heat shock protein (Hsp10) involves the mobile loop and attenuation of the Ras GTP-ase pathway
Authors:	Lin, KM;Hollander, JM;Kao, VY;Lin, B;Macpherson, L;Dillmann, WH
Contributors:	Division of Medical Engineering Research
Abstract:	Heat shock proteins (hsp), hsp60 and hsp10, are involved in the folding of imported mitochondrial proteins and the refolding of denatured proteins after stress. We examined whether hsp10 can reduce myocyte death by its mitochondrial function or by interacting with cytoplasmic signaling pathways. Overexpression of hsp10 by adenoviral infection decreased myocyte death induced by hydrogen peroxide, sodium cyanide, and simulated ischemia and reoxygenation (SI/RO). We generated an adenoviral vector coding for a temperature-sensitive mutant hsp10 protein (P34H), incapable of cooperatively refolding denatured malate dehydrogenase with hsp60. Overexpression of the hsp10 mutant potentiated SI/RO-induced myocyte death. Analysis of electron transport chain function revealed increased Complex I capacity with hsp10 overexpression, whereas hsp10( P34H) overexpression decreased Complex II capacity. Hsp10 overexpression preserved both Complex I and II function after SI/RO. Examination of the Ras GTP-ase signaling pathway indicated that inhibition of Ras was required for protection by hsp10. Constitutive activation of Ras abolished the effects afforded by hsp10 and hsp10(P34H). Hsp10 overexpression inactivated Raf, ERK, and p90Ribosomal kinase (p90RSK) before and after SI/RO. Our results suggest that complex mechanisms are involved in the protection by hsp10 against SI/RO-induced myocyte death. This mechanism may involve the hsp10 mobile loop and attenuation of the Ras GTP-ase signaling pathway.
Keywords:	Biochemistry & Molecular Biology;Biology;Cell Biology
Date:	2004-04
Relation:	FASEB Journal. 2004 Apr;18(6):1004-1006.
Link to:	http://dx.doi.org/10.1096/fj.03-0348fje
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0892-6638&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000221108800018
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=6944240429
Appears in Collections:	[Kurt Ming-Chao Lin] Periodical Articles

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