國家衛生研究院 NHRI:Item 3990099045/14652
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    Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/14652


    Title: Protodioscin inhibits bladder cancer cell migration and growth, and promotes apoptosis through activating JNK and p38 signaling pathways
    Authors: Chen, YR;Wang, SC;Huang, SP;Su, CC;Liu, PL;Cheng, WC;Chuu, CP;Chen, JK;Bao, BY;Lee, CH;Ke, CC;Wu, HE;Chang, HH;Yeh, HC;Li, CY
    Contributors: Institute of Cellular and Systems Medicine;Institute of Biomedical Engineering and Nanomedicine
    Abstract: Bladder cancer is one of the most common malignancies of the male genitourinary urinary system. Protodioscin is a steroidal saponin with anti-cancer effects on several types of cancers; however, the anti-cancer activities of protodioscin on bladder cancer have not yet been investigated. Therefore, we aimed to examine the anti-cancer effects of protodioscin on bladder cancer. Two types of bladder cancer cell lines, non-muscle-invasive 5637 cells and muscle-invasive T24 cells, were used to evaluate the effects of protodioscin on cell growth, migration, invasion and epithelial-mesenchymal transition(EMT) marker expressions. Transcriptome analysis was performed by RNA-seq and validated using real-time PCR and western blot; additionally, an in vivo xenograft animal model was established and the anti-tumor effects of protodioscin were tested. Our results demonstrated that protodioscin inhibited cell proliferation, migration, motility and invasion on 5637 and T24 cells. Additionally, protodioscin also induced cell apoptosis and arrested the progression of cell cycle at G2 phase in bladder cancer cells. Moreover, protodioscin inhibited EMT through increased protein expression of E-cadherin and decreased protein expression of N-cadherin and vimentin. RNA-seq analysis indicated that protodioscin regulated mitogenactivated protein kinase(MAPK) and phosphoinositide 3-kinases(PI3K)/protein kinase B(AKT)/mammalian target of rapamycin(mTOR) signaling pathways as further verified by Western blot. Furthermore, protodioscin
    Date: 2022-12
    Relation: Biomedicine and Pharmacotherapy. 2022 Dec;156:Article number 113929.
    Link to: http://dx.doi.org/10.1016/j.biopha.2022.113929
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0753-3322&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000884780200002
    Cited Times(Scopus): https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85140807302
    Appears in Collections:[Chih-Pin Chuu] Periodical Articles
    [Jen-Kun Chen] Periodical Articles

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