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Please use this identifier to cite or link to this item:
http://ir.nhri.org.tw/handle/3990099045/14836
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| Title: | Experimental verification for numerical simulation of thalamic stimulation-evoked calcium-sensitive fluorescence and electrophysiology with self-assembled multifunctional optrode |
| Authors: | Liang, YW;Lai, ML;Chiu, FM;Tseng, HY;Lo, YC;Li, SJ;Chang, CW;Chen, PC;Chen, YY |
| Contributors: | NHRI Graduate Student Program |
| Abstract: | Owing to its capacity to eliminate a long-standing methodological limitation, fiber photometry can assist research gaining novel insight into neural systems. Fiber photometry can reveal artifact-free neural activity under deep brain stimulation (DBS). Although evoking neural potential with DBS is an effective method for mediating neural activity and neural function, the relationship between DBS-evoked neural Ca(2+) change and DBS-evoked neural electrophysiology remains unknown. Therefore, in this study, a self-assembled optrode was demonstrated as a DBS stimulator and an optical biosensor capable of concurrently recording Ca(2+) fluorescence and electrophysiological signals. Before the in vivo experiment, the volume of tissue activated (VTA) was estimated, and the simulated Ca(2+) signals were presented using Monte Carlo (MC) simulation to approach the realistic in vivo environment. When VTA and the simulated Ca(2+) signals were combined, the distribution of simulated Ca(2+) fluorescence signals matched the VTA region. In addition, the in vivo experiment revealed a correlation between the local field potential (LFP) and the Ca(2+) fluorescence signal in the evoked region, revealing the relationship between electrophysiology and the performance of neural Ca(2+) concentration behavior. Concurrent with the VTA volume, simulated Ca(2+) intensity, and the in vivo experiment, these data suggested that the behavior of neural electrophysiology was consistent with the phenomenon of Ca(2+) influx to neurons. |
| Date: | 2023-02-13 |
| Relation: | Biosensors. 2023 Feb 13;13(2):Article number 265. |
| Link to: | http://dx.doi.org/10.3390/bios13020265 |
| JIF/Ranking 2023: | http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=2079-6374&DestApp=IC2JCR |
| Cited Times(WOS): | https://www.webofscience.com/wos/woscc/full-record/WOS:000938463300001 |
| Cited Times(Scopus): | https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85148964646 |
| Appears in Collections: | [其他] 期刊論文
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| PUB36832031.pdf | | 6066Kb | Adobe PDF | 214 | View/Open |
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