國家衛生研究院 NHRI:Item 3990099045/14838
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    Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/14838


    Title: Antimicrobial peptide mastoparan-AF Kills multi-antibiotic resistant escherichia coli O157:H7 via multiple membrane disruption patterns and likely by adopting 3-11 amphipathic helices to favor membrane interaction
    Authors: Lin, CH;Shyu, CL;Wu, ZY;Wang, CM;Chiou, SH;Chen, JY;Tseng, SY;Lin, TE;Yuan, YP;Ho, SP;Tung, KC;Mao, FC;Lee, HJ;Tu, WC
    Contributors: National Mosquito-Borne Diseases Control Research Center
    Abstract: We investigated the antimicrobial activity and membrane disruption modes of the antimicrobial peptide mastoparan-AF against hemolytic Escherichia coli O157:H7. Based on the physicochemical properties, mastoparan-AF may potentially adopt a 3-11 amphipathic helix-type structure, with five to seven nonpolar or hydrophobic amino acid residues forming the hydrophobic face. E. coli O157:H7 and two diarrheagenic E. coli veterinary clinical isolates, which are highly resistant to multiple antibiotics, are sensitive to mastoparan-AF, with minimum inhibitory and bactericidal concentrations (MIC and MBC) ranging from 16 to 32 μg mL(-1) for E. coli O157:H7 and four to eight μg mL(-1) for the latter two isolates. Mastoparan-AF treatment, which correlates proportionally with membrane permeabilization of the bacteria, may lead to abnormal dents, large perforations or full opening at apical ends (hollow tubes), vesicle budding, and membrane corrugation and invagination forming irregular pits or pores on E. coli O157:H7 surface. In addition, mRNAs of prepromastoparan-AF and prepromastoparan-B share a 5'-poly(A) leader sequence at the 5'-UTR known for the advantage in cap-independent translation. This is the first report about the 3-11 amphipathic helix structure of mastoparans to facilitate membrane interaction. Mastoparan-AF could potentially be employed to combat multiple antibiotic-resistant hemolytic E. coli O157:H7 and other pathogenic E. coli.
    Date: 2023-02-20
    Relation: Membranes. 2023 Feb 20;13(2):Article number 251.
    Link to: http://dx.doi.org/10.3390/membranes13020251
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=2077-0375&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000940509700001
    Cited Times(Scopus): https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85149127390
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